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KMID : 0380020040190010027
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 1 p.27 ~ p.32
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Production and Process Monitoring of 5-Aminolevulinic Acid (ALA) by Recombinant E coli 2. Process Monitoring by a 2-Dimensional Fluorescence Sensor
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Lee Jong-Il
Jeong Sang-Yoon
Lim Yong-Sik
Chung Sang-Wook
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Abstract
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2-Dimensional fluorescence sensor has a wide range of excitation and emission wavelengths, that some biogenic fluorphors in a biological process can be monitored simultaneously. The production processes of 5-aminolevulinic aicd (ALA) by recombinant E. coli BL21 (DE3) pLysS harboring plasmid pFLS45 were on-line monitored by a 2-dimensional fluorescence sensor The characteristics of fluorescence spectrum was dependent upon physical and biological factors of a bioprocess such as culture pH, cell mass etc. Some off-line data were correlated to the fluorescence intensity well, which was monitored at some combination of excitation and emission wavelengths by the 2-dimensional fluorescence sensor
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KEYWORD
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2-Dimensional fluorescence sensor, ALA production, recombinant E. coli
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